Circ_0086720 knockdown strengthens the radiosensitivity of non-small cell lung cancer via mediating the miR-375/SPIN1 axis

Radioresistance is an important cause of cancer treatment failure. Circular RNAs (circRNAs) play crucial roles in development, including the radioresistance. This research aimed to determine function and related mechanism circ_0086720 radioresistance non-small cell lung (NSCLC). The expression circ_0086720, miR-375, Spindlin 1 (SPIN1) was measured using a quantitative real-time polymerase chain reaction (qRT-PCR). Cell survival fraction analyzed colony formation assay, apoptosis monitored by flow cytometry assay. activities caspase 3 9 were assessed corresponding commercial kits. protein levels SPIN1 γH2AX detected western blot. Bioinformatics analysis performed predict targets miR-375. Dual-luciferase reporter RNA immunoprecipitation (RIP) pull-down assay conducted validate interaction between miR-375 or SPIN1. animal model constructed ascertain role vivo. increased radioresistant NSCLC tissues, while decreased. knockdown sensitized cells radiation further inhibit induce apoptosis. Circ_0086720 targeted suppressed expression, bound impair expression. deficiency overexpression could attenuate knockdown-mediated radiosensitivity. also enhanced radiosensitivity block tumor growth To conclude, downregulation sensitivity regulating miR-375/SPIN1 axis, contributing improvement radiotherapies NSCLC.